• Principle : spin column (silica membrane)
• Sample size : 150 µl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant 
• Fragment size : 100 bp ~30 kb
• Recovery rate : 80 ~ 90 %
• Binding capacity : 30 ug
• Elution volume : 40 ~ 50 µl 
• Operation time : 20 min

Important Notes:

• Make sure everything is RNase-free when handling this system.
• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Add 1 ml of VNE Buffer to the tube of lyophilized Carrier RNA, mix well by vortexing and transfer the mixture to the VNE Buffer when first open. Store the Carrier RNA added VNE Buffer at 4 °C.
• Add required ethanol (96-100%) to Wash Buffer 1 and Wash Buffer 2 before use.
• Preheat RNase-free water to 70?C for elution step.

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APVNK050/ APVNK100/ APVNK200

1. VNE Buffer    35ml/ 70ml/ 140ml
2. Wash Buffer 1 * (concentrate).   22ml/ 44 ml/ 88ml
3. Wash Buffer 2 * (concentrate).   20ml/ 40ml/ ml / 40ml x 2
4. RNase-free Water      6 ml/ 6ml x2/ 20ml
5. Carrier RNA.   0.4 mg/ 0.8 mg/ 1.6 mg
6. VNE Column.   50pcs/ 100pcs/ 200pcs
7. Collection Tube.  100pcs/ 200pcs/ 400pcs
8. Elution Tube.   50pcs/ 100pcs/ 200pcs 
9. User Manual x 1