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• Principle : mini spin column (silica matrix)
• Sample size : 300 μl
• Operation time : 30 ~ 60 minutes
• Binding capacity : up to 100 μg total RNA / column
• Expected yield : 1 μg
• Column applicability : centrifugation and vaccum
• Minimum elution volume : 40 μl

Important Notes :

• Make sure everything is RNase-free when handling RNA.
• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood. 
• Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
• Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.

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1. RL Buffer    120ml/ 240ml
2. RB Buffer    25ml/ 45ml
3. Wash Buffer 1    30ml/ 60ml
4. Wash Buffer 2 * (concentrate)    15ml/ 35ml
5. RNase-free Water     6ml/ 6ml
6. Filter Column    50pcs/ 100pcs
7. RB Mini Column    50pcs/ 100pcs
8. Collection Tube    100pcs/ 200pc
9. Elution Tube4     50pcs/ 100pcs
10. User Manual x1