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• Principle : mini spin column (silica matrix)
• Sample size : up to 100 mg plant tissue or 1 x10⁷ plant cellOperation time : 30 ~ 60 minutes
• Binding capacity : up to 100 μg total RNA / column
• Expected yield : 5 ~ 30 μg of total RNA from 100 mg of young leave
• Column applicability : centrifugation and vaccum
• Minimum elution volume : 30 μl

Important Notes :

• Make sure everything is RNase-free when handling RNA.
• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Caution: ß-mercaptoethanol is hazardous to human health. Perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood.
• Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
• Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.

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APBRK050, APBRK100

1. RL Buffer.   120ml/ 240ml
2. RB Buffer.   25 ml/ 45ml
3.  Wash Buffer1    30ml/ 60ml
4. Wash Buffer 2 * (concentrate).    15ml/ 35ml
5. RNAse-free Water     6ml/ 6ml
6. Filter Column.   50pcs/ 100pcs
7. Mini Column.    50pcs/ 100pcs
8. Collution Tube    100pcs/ 200pcs
9. Elution Tube    50pcs/ 100pcs
10. Useer Manual x1