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      ¹ï°ª«~½èªº°í«ù¬O ÁÉ®¦´µ¥Íª«¬ì§Þ¤½¥q³Ì¤Þ¥H¬°¶Æªº«ü¼Ð¡A§Ú­Ì³£¯àÁA¸Ñ«È¤á±ýÁʱo°Ó«~µ´¹ï·|­n¨D¦nªº«~½è ¤Î³Ì¤pªº§ë¸ê¡A°ò©ó¦¹¥»¤½¥q¿ï¾Ü»P¥[®³¤j BioBasic ¤½¥q¦X§@¡A¤Þ¶i°ª¬ì§Þ§Þ³N¤Î°ª«~½è¤Þ¤l¦X¦¨ªºªA°È¡ABioBasic ¤½¥q¬OÄÝ¥þ²yª¾¦W¥ç³Ì¤jªº¤Æ¾Ç¦X¦¨ DNA ¼t°Ó¤§¤@¡A¹ê¤O¶¯«p¡A¾Ö¦³ 10 ¥x¥H¤W ABI 3900 °ª³q¶q DNA ¦X¦¨»ö¡A¬°·í¤µ³Ì¥ý¶iªº»ö¾¹¤§¤@¡A¨C¤é¤Þ¤l²£¶q¶W¹L 6000 ±ø¥H¤W¡C  HAP ¬O High Affinity Purification ªºÁY¼g¡A¬O BioBasic ¤½¥q¬ãµo¦X¦¨  Oligo ªº¯S®í§Þ³N¡A¨Ã¥i¨ú¥N²{¦æ De-salted Oligo ¼Ð·Ç¤Î OPC¦X¦¨¤è¦¡¥B¯Â«× > 98% ¥H¤W»P HPLC ¯Â«×¬Û·í¦ý©M De-salt Oligo »ù®æ¤@¼Ë«K©y¡C¨ä­ì²z¬O±N Oligo ¦X¦¨­ì²G³q¹L HAP Column¡A§t DMT«OÅ@°òªº§¹¾ã Oligo    (DMT-ON-Oligo)±N·|¿ï¾Ü©Ê¦a»P Resin µ²¦X¡A¦Óªø«×¸ûµu¤£§¹¾ãªº Oligo ´N·|ª½±µ³q¹L Column ¦Ó¥h°£¡A³Ì«á¦b·L»ÄªºÀô¹Ò¤U²¾°£ DMT«O Å@°ò¦Ó±o¨ì§¹¾ãªºOligo¡C

     HAP ¬°¥[®³¤jBioBasic ¤½¥q¿W¥ß¬ãµoªº¤@­Ó·s§Þ³N¡A¨ã¦³§Ö³t¡B²³æ¡B¯Â«×°ªµ¥ÀuÂI¡A²`¨ü¤¤°ê¤j³°¡B¬ü°ê¡B¤é¥»¡BÁú°ê¡B·s¥[©Y¡B¦L«×¡B°¨¨Ó¦è¨È¡B®õ°êµ¥°ê®a¨Ï¥ÎªÌÅwªï »á¨ü¦nµû¡A¬O±z Modified Base ¦X¦¨ªº³Ì¨Î¤è¦¡¡C

¥|¤jÀuÂI :

1. §ó°ª¯Â«× : ¯Â«×¥i¤j©ó 98%, »·¤j©ó de-salted oligo ( ¥u¦³ 30% : 60-mer ¨ì 68%: 20-mer )

2. Modified Base¦X¦¨ªº³Ì¨Î¤è¦¡ : HAP¾A¦X¤@¯ë¤Îmodified base ªº¦X¦¨, ¤ñ HPLC «K©y§Ö³t, ¦Ó PAGE ®Ú¥»¤£¯à ¯Â¤Æmodified base oligo.

3. ¸gÀÙ¹ê´f : »PHPLC¬Û¦P¯Â«×¦ý©Mdesalt¤@¼Ë«K©y.

4. ¹qªa¤ÀªR¹Ï¤ÎMASS QC ªº½T©w : ¨C±øprimer­ì¼t³£¦³¯d¤U¤Ö³\¼Ë«~, ¨Ï¥Î¦p¦³°ÝÃD, ¥i­n¨D­ì¼t°µMASS QC. ¦¹ºØ MASS QC ªº´ú©w¬O¨Ì primer ªº¤À¤l¶q¦Óºë½Tªº§PŪ primer ¦X¦¨§Ç¦Cªº¥¿½T©Ê, ¨ä»~®t²v¥u¦³ 0.02-0.03% ¥[¤W¨C±øprimer data sheet¤W³£¦³¹qªa¤ÀªR, ¨Ï±oprimer ¦X¦¨ªº·Ç½T©Ê¥i¥H±o¨ì«OÃÒ, «È¤á¦p¦³¨Ï¥Î¤W°ÝÃD, ¤]¥i¥H±o¨ì¸Ñ¨M !

* §Ú­Ì¤]±µ¨ü¯S®í¼Ð°O­×¹¢¤Þ¤l¦X¦¨¦³Ävª§¤O»ù®æÅwªï¤Wºô¬d¸ß©Î¨Ó¹q¬¢¸ß¡C

    
 
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  2 O.D / 5 O.D / 10 O.D / 20 O.D / 40 O.D / 100 O.D
¯Â¤Æ¤è¦¡ > 61 mer ¤£«ØÄ³°µ HAP ¦X¦¨
 HAP¡G¾A¥Î©ó PCR ©Î©w§Ç¹êÅç¡C < 60 mer
 PAGE¡G¾A¥Î©óªøÃì PCR ©Î©w§Ç¹êÅç¡C
 HPLC¡G¾A¥Î©ó PCR¡B©w§Ç©Î§@¬°±´°w¥Î³~¡C
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B NOT A
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Y T/C
D NOT C
S G/C

¼Ð©w¤À¤l«¬¦¡ ¼Ð©w¦ì¸m
Amine Modifier 3' , 5'
Biotin 3' , 5'
Cy3 or Cy5 3' , 5'
Dabcyl 3' , 5'
Dig any
dU or dI any
FAM 3' , 5'
FITC any
Phosphorylation 3' , 5'
Phosphrothiated any
TAMRA or Rox 3' , 5'
HET or HEX 3' , 5
SH or HS-SH 3' , 5'
Thiol Modifier 3' , 5'
 ¯S®í¼Ð°O­×¹¢
 ²V¦X®Ö»Ä¡G ¤@­Ó Base ¦ì¸m¥i²V¦X 2 - 4 ºØ§Ç¦C¡A½Ð°Ñ¾\²V¦X®Ö»Ä¹ï·Óªí¡C
 ³æ¤@­×¹¢¡G ¦³¦hºØ¼Ð©w©Î¿Ã¥ú­×¹¢¡A¦ì¸m¦³ 3'¡B5' ©Î¥ô·N¦ì¸m¡A½Ð°Ñ¦Ò¼Ð©w­×¹¢ªí¡C
 Âù­«¼Ð©w¡G ¦b§Ç¦C¨âºÝ¼Ð©w¿Ã¥ú¤À¤l¡A¦³ TaqMan Probe ( 5' FAM - 3' TAMRA ) ©M Molecular Beacon ( 5' FAM - 3' Dabcyl ) ¨âºØ¡C


 

¤Þ¤l¦X¦¨ Primer ( Oligo ) Synthesis ºô¸ô­qÁʳ栠                                 «È¤á°ò¥»¸ê®Æ

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Primer ¦WºÙ»P§Ç¦C¡G( ¦WºÙ»P§Ç¦C¤å¦r¿é¤J¥iµL­­©µªø ¡A¦pªí®æ¤£¨¬½Ð°e¥X«á¦A¶ñ¤@¦¸­q³æ )

       ¦WºÙ

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HAP     HPLC     PAGE
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1 O.D     2 O.D     5 O.D     8 O.D     10 O.D    20 O.D    25 O.D     30 O.D     40 O.D    100 O.D
¯S®í¼Ð°O³B²z¡G * ¦¹¿ï¶µ¦p¦³»Ý­n½Ð¬¢·~°È¬°§A¸Ñ»¡
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5'ºÝ           ³B²z¤è¦¡
3'ºÝ           ³B²z¤è¦¡
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¨âºÝ¼Ð°O¡G
5'FAM--------3'Dabcyl
5'FAM--------3'TAMRA
 

ª`·N¨Æ¶µ :    * HAP Primer ­qÁÊ«á¹w­p 3 ~ 4  ¤Ñ¥æ³f, ¤¤«n³¡¦h¥[¤@¤Ñ, ¯S®íLabel ¦X¦¨ ( PAGE / HPLC / ¨ä¥¦ )

                       ¬ù5 ~ 7¤Ñ¥æ³f

1. ½Ð©ó·í¤Ñ¤U¤È¤­ÂI«e¤U­q³æ¡C
2. §Ç¦C®Ñ¼g½Ð¥H¤p¼g "g" ¥N´À¤j¼g "G"¡F¤Þ¤l¦WºÙ¤Î§Ç¦C¤¤¤Á¤Å§tªÅ¥Õ¦r¤¸¡C
3. §Ç¦C¤¤­Y¦³²V©M®Ö»Ä (mixed base)¡A½Ð¥H¯S©w­^¤å¦r¥À¥N´À¡C
4. ¶ñ¼g§¹²¦·Ð½Ð«ö¤U°e¥XÁä¡A¦pªG¦¨¥\±H¥X¡A§A±N·|¦¬¨ì email ½T»{«H¨ç¤Î­q³æ¸¹½X¡A¦p¥¼¦¬¨ì½Ð¹q¬¢¥»¤½¥q¡C

  
 


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