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• Principle : mini spin column (silica matrix)
• Sample size : 

1. Up to 25 mg animal tissue
2. 1.2 cm mouse tail
3. Up to 10⁷ cultured cells

• Operation time : 30 ~ 60 minutes
• Binding capacity : up to 60 µg total DNA / column
• Expected yield : 15 ~35 μg / prep
• Column applicability : centrifugation and vaccum
• Minimum elution volume : 50 μl

Important Notes :

• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ¢J .
• Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
• Prepare dry baths or water baths before the operation. One to 60¢J for step 2.1 and the other to 70¢J for step 2.2.
• Preheat the Elution Buffer to 70¢J for step 7.
• All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.

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1. TG1 Buffer   15 ml/ 30ml/ 60ml
2. TG2 Buffer.  15 ml/30 ml/ 60ml
3. Proteinase K (lyophilized).  11 mg/ 11mg x 2/ 11mg x 4
4. GW Buffer (concentrate).   22 ml/ 44 mg/ 88 ml
5. Wash Buffer (concentrate)    10ml/ 20ml/ 40ml 
6. Elution Buffer.  15ml/ 30ml/60ml
7. TG Mini Column.  50 pcs/ 100pcs/ 200 pcs
8. Collection Tube.  100 pcs/ 200/ 400 pcs
9. Elution Tube.  50 pcs/ 100 pcs/ 200 pcs 
10. User Manual x1