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• Principle : mini spin column (silica
• Sample size : 1 ~ 3 ml
• Size of plasmid or construct : < 15 kb
• Operation time : < 25 minutes
• Typical Yield : 20 ~ 30 μg
• Binding capacity : 60 μg/ column
• Column applicability : centrifugation and vaccum

Important Notes:

• Store RNase A at -20 °C upon recipit of kit
• Add 0.5 ml of PD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the PD1 bottle, mix well by vortexing and store the PD1 buffer at 4 °C.
• If precipitates have formed in PD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
• Preparation of Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
• Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.

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1. PD1 Buffer.    13ml/ 25ml/ 75ml
2. PD2 Buffer.   13ml/ 25ml/ 75ml
3. PD3 Buffer.   18 ml/ 35ml/ 10ml. 
4. PDW Buffer.   23ml/ 45ml/ 135ml 
5. Wash Buffer (concentrate)     10ml/ 20ml/ 50ml
6. Elution Buffer    7ml/ 15ml/ 35ml
7. PD Column.   50pcs/ 100pcs/ 300pcs
8. Collection Tube.    50pcs/ 100pcs/ 300pcs
9. RNase A (Lyophilized).   1.25 mg/ 2.5mg/ 7.5 mg 
10. User Manual x1