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• Principle : mini spin column (silica matrix)
• Sample size : 

1. Wet weight ¡Ø 100 mg
2. Dry weight ¡Ø 20 ml

• Operation time : 30 ~ 60 minutes
• Binding capacity : up to 60 μg total DNA / column
• Expected yield : 5 ~ 40 μg /prep
• Column applicability : centrifugation and vaccum
• Minimum elution volume : 50 μl

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APPGK050/ APPGK100

1. PG1 Buffer.   25ml/ 55ml
2. PG2 Buffer.   8ml/ 15ml
3. PG3 Buffer (concentrate).  15 ml/ 30ml
4. GW Buffer (concentrate).  13ml/ 26ml
5. Wash Buffer (concentrate)    15ml/ 30ml
6. Elution Buffer.  15ml/ 30ml
7. RNase A (lyophilized).  22mg/ 43 mg
8.  Filter Column.  50 pcs/ 100pcs
9. PG Column.  50 pcs/ 100 pcs
10. Collution Tube.  100 pcs/ 200 pcs
11. User Manual x1

 Important Note

• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Check PG1 Buffer before use, Warm PG1 Buffer at 60¢J for 5 min if any precipitate formd.
• Preheat dry baths or water baths to 65¢J before the operation.
• Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.
• Store RNase A at -20¢J upon recipit of kit. Add sterile ddH₂O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20¢J .
• All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.