Pfu DNA polymerase is isolated from the Pyrococcus furiosus. The multifunctional thermostable enzyme possesses both of 5’ to 3’-DNA polymerase and 3’-to 5’- exonuclease activity, which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerases. Pfu DNA polymerase has a temperature optimum between 72oC and 78oCremains more than 95% active following one hour incubation at 95oC
實驗數據
GeneMark’s Pfu
DNA polymerase can amplify products up to 3 kb in length
