Preparation of high quality total RNA from variable sources.
Simple on-column DNase I treatment for genomic DNA removal.
High yields up to 100 £gg.
No phenol / chloroform extraction, no ethanol precipitation.
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The RNA purified from supplier Q contains large amount of genomic DNA contamination. RT-PCR of zebrafish EF-1£\ gene. RNA were purified by kits from GeneMark and Q supplier. In the control reaction that add Taq polymerase only with the RNA products, genomic DNA contamination was observed from Q supplier.
Non-denaturing gel electrophoresis of the total RNA purified from various samples by GeneMark and Q supplier.