High signal sensitivity and long signal duration for chemiluminescent
LumiFlash™ Ultima Chemiluminescent Substrate, HRP is an advanced ECL product in terms of efficacy and sensitivity for chemiluminescent detection of immobilized proteins (Western blotting and ELISA). LumiFlash™ Ultima Chemiluminescent Substrate, HRP provides high sensitivity and long signal duration in Western blotting application. It needs very short exposure time to the X-ray film or other documentation systems, and can get low background and high signal with clear image. LumiFlash™ Ultima Chemiluminescent Substrate, HRP is the perfect choice for most Western blotting application.
Special Characteristic
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High signal sensitivity – detection of target protein at low-picogram level on WB and ELISA application
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Antibody saving – diluted lower antibody concentration for WB and ELISA application
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Long duration – provide stable signal for long exposure
- Economy – cost less than other ECL substrate with similar sensitivity level
Product Detail :
Figure 1. The comparison of WB application for LumiFlash Ultima, VisGlow Plus and Luminata Crescendo.
K562 cell lysate with 1/2 serial dilution from 50 µg was separated by 12.5% SDS-PAGE and probed by anti HSP 47. All results were exposed to X-ray film for 3 minutes.
Order Information
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Cat. No.
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Product Name
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Description
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LF08- 500
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LumiFlash™ Ultima Chemiluminescent
Substrate, HRP
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250mL soultion A +
250mL solution B
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Reference:
1. Kim, Yunho, et al. "Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis." Nature communications 7 (2016): 10347.
2. Lim, Chae-Seok, et al. "PKCα-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory." Scientific reports 7.1 (2017): 1-15.
3. Lee, I-Ta, et al. "Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells." Cytokine 113 (2019): 380-392.
4. Cho, Min Young, et al. "Induction of DEAD Box helicase 5 in early adipogenesis is regulated by Ten-eleven translocation 2." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2020): 158684.
