- Very High signal sensitivity: detection of target protein at the low-femtogram level
- Long duration: signal duration up to 12 hours)
- Long shelf life: 18 months long at 4℃
Order Information:
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Cat. No.
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Product Name
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Description
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LF24-100
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LumiFlash™ Femto Chemiluminescent Substrate, HRP System
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50mL Solution A + 50mL Solution B
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Product Detail:
Figure 1. Western blotting image of LumiFlash™ Femto Chemiluminescent Substrate, HRP System.
Hela cell lysate with 1/2 serial dilution from 7 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked for 1 min at room temperature with BlockPRO™ 1 Min (#BM01-500). (A) The blot was probed at 1:2,500 with Anti-Akt antibody (#4691, Cell Signaling Technology). (B) The blot was probed at 1:10,000 with Anti-GAPDH antibody (#ab8245, Abcam). A HRP-conjugated secondary antibody was applied and developed with LumiFlash™ Femto Chemiluminescent Substrate (#LF24-100). All blots were simultaneously exposed for 10 seconds, 30 seconds, and 60 seconds using ChemLux SPX-600 imaging system.
LumiFlash™ Series:
