|
GM/ RP PCR 系列相關產品 |
人氣指數 |
1872 |
|
 |
| 型號：GM/ RP |
| 中文名稱：PCR 系列相關產品 |
| 英文名稱： |
| 廠牌：GeneMark |
| 供應商：賽恩斯生物科技股份有限公司 |
|
|
|
|
|
|
|
商 品 說 明 資 料 |
 |
- First Stranded cDNA Synthesis Kit
DNA Polymerases
|
Product Name
|
GenTaq DNA Polymerase
|
GenTaq Plus DNA Polymerase
|
GenHot Taq DNA Polymerase
|
GenFast Taq DNA Polymerase
|
gmPfu DNA Polymerase
|
LongFast Pfu DNA Polymerase
|
GenBest Taq DNA Polymerase
|
|
Polymerase
|
GenTaq
|
GenTaq + Pfu
|
Taq + Mab
|
Modified Taq
|
Modified Pfu
|
Modified Pfu
|
Modified Pfu
|
|
Proofreading
|
No
|
Yes
|
No
|
No
|
Yes
|
Yes
|
Yes
|
|
Product Overhang
|
3’-A
|
3’-A
|
3’-A
|
3’-A
|
Blunt
|
Blunt
|
Blunt
|
|
Extension
|
1 min/kb
|
1 min/kb
|
1 min/kb
|
15 sec/kb
|
1 min/kb
|
15-30 sec/kb
|
10~30 sec/kb
|
|
PCR product size
|
Up to 14 kb
|
Up to 14 kb
|
Up to 6 kb
|
Up to 15 kb
|
Up to 10 kb
|
Up to 10 KB
|
Up to 20 KB
|
|
Application
|
Detection
|
Detection Cloning Expression
|
Detection Multiplex PCR
|
Fast PCR Detection Multiplex PCR
|
Cloning Expression Mutagenesis
|
Fast PCR Cloning Expression Mutagenesis
|
Fast PCR Cloning Expression Mutagenesis
|
|
|
產品名稱
|
產品編號
|
包裝
|
價格($NT)
|
|
PCR Polymerase
|
GenTaq DNA Polymerase
|
GM008
|
1000 U (2U/ul or 5U/ul)
|
1890
|
|
GenTaq Plus DNA Polymerase
|
GM008P
|
250 U
1000 U
|
2048
8190
|
|
GenHot Taq DNA Polymerase
|
GM008HS
|
200 U
1000 U
|
1800
7200
|
|
GenFast Taq DNA Polymerase
|
GM008F
|
200 U
1000 U
|
1200
4800
|
|
gmPfu DNA Polymerase
|
GPU425
|
250U
500U
|
1890
3402
|
|
LongFast Pfu DNA Polymerase
|
GPU4
|
200U
1000U
|
3200
13200
|
|
GenBest Taq DNA Polymerase
|
GM008B
|
200U
1000U
|
2400
9600
|
|
|
產品名稱
|
產品編號
|
包裝
|
價格($NT)
|
|
PCR Master Mix
|
PCR Master Mix (2X)
|
RP02
|
400r
2000r
|
2079
8316
|
|
PCR Master Mix II
|
RP02 II
|
400r
2000r
|
2079
8316
|
|
PCR Plus Master Mix II
|
RP02-Plus-II
|
100r
|
2394
|
|
PCR Pfu Master Mix II
|
RP02-pfu-II
|
200r
|
2520
|
|
PCR Hot-Start Master Mix II
|
RP02HS-II
|
200r
|
3150
|
|
PCR Dye Master Mix
|
PCR Dye Master Mix II (2X)
|
RP02D-II-B
|
200r
1000r
|
1155
4620
|
|
PCR Dye Master Mix II (5X)
|
RP02D-II-A
|
200r
1000r
|
1155
4620
|
|
2X GenFast PCR MasterMix, 1ml
|
RP02D-Fast
|
100r
500r
|
1800
9000
|
|
PCR Dye Plus Master Mix
|
RP02D-Plus
|
100r
|
2394
|
|
PCR Dye Pfu Master Mix
|
RP02D-pfu
|
200r
|
2520
|
|
PCR Dye Hot-Start Master Mix
|
RP02D-HS
|
200r
|
3150
|
|
Cat. No.
|
Product
|
Sample
|
DNA Yield
|
Format
|
Operation Time
|
|
RP02-LD300
|
SAMtissuet Direct PCR Kit
|
cells, mouse tails, liver,muscle, hair shafts, skin, saliva and other animal tissues
|
For PCR
|
no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
|
15 min
|
|
RP02-LD301
|
SAMbacteria Direct PCR Kit
|
both gram-positive and gram-negative (genomic DNA and plasmid DNA)
|
For PCR
|
no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
|
-
|
|
RP02-LD303
|
SAMseed Direct PCR Kit
|
different kinds of seeds ; Arabidopsis, carrot, corn, cucumber, pepper, soybean, turnip, wheat and more
|
For PCR
|
no DNA purification needed ; Uses pre- 2X PCR mix( containing dye)
|
15 min
|
|
RP02-LD304
|
SAMblood Direct PCR Kit
|
blood, saliva, other body, fluids, buccal cells on swabs, Cultured cells, and other liquid cells
|
For PCR
|
no DNA purification ; Uses pre-optimized 2X PCR mix( containing dye)
|
15 min
|
|
RP02-PD8
|
GMplant Direct PCR Kit
|
plant leaves
|
For PCR
|
no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
|
-
|
|
產品名稱
|
產品編號
|
包裝
|
價格($NT)
|
|
SAMtissue Direct PCR Kit
|
RP02-LD300
|
100r
|
5460
|
|
SAMbacteria Direct PCR Kit
|
RP02-LD301
|
100r
|
5460
|
|
GMplant Direct PCR Kit
|
RP02-PD801
|
100r
|
5460
|
|
SAMseed Direct PCR Kit
|
RP02-LD303
|
100r
|
5460
|
|
SAMblood Direct PCR Kit
|
RP02-LD304
|
100r
|
5460
|
Reverse transcription-PCR (RT-PCR)
> Transcriptase 反轉錄?
> 一步法RT-PCR
> 兩步法RT-PCR
|
|
AMV
|
MMLV
|
|
來源
|
Avian
|
Murine
|
|
最適作用溫度
|
42℃ (可有效解開二級結構)
|
37℃
|
|
RNase H
|
強
|
弱
|
|
合成長度
|
短
|
長
|
** MMLV reverse transcription 改良版 → 提高溫度耐受性
|
|
SuperSAMscript III Reverse Transcriptase
|
SuperSAMscript IV Reverse Transcriptase
|
|
作用溫度
|
37℃~55℃ (適用於具有複雜二級結構之RNA)
|
42℃~65℃ (適用於具有複雜二級結構之RNA)
|
|
合成片段大小
|
100bp~15kb（無RNase H活性, 可增加transcripts產量）
|
100bp~12kb (適合長片段cDNA合成)
|
|
模板量
|
Total RNA: 1 ng-5 μg mRNA: 1 ng-250 ng Specific RNA: 0.01 pg-0.5 μg
|
Total RNA 10 ng~5 μg mRNA 1ng~500 ng Specific RNA: 0.01 pg-0.5 μg
|
First-Stranded cDNA Synthesis Kit
|
Product Name
|
Cat. No.
|
Package
|
Price
|
|
SuperSAMscript III First Stranded cDNA Synthesis Kit
|
GRS03M
|
50r
|
$3000
|
|
SuperSAMscript IV First Stranded cDNA Synthesis Kit
|
GRS04M
|
50r
|
$3600
|
One-Step RT-PCR Kit
|
Product Name
|
Cat. No.
|
Package
|
Price
|
|
One-Step RT-PCR Kit III
|
RP01-III
|
50r/250r
|
$3900/$16580
|
|
One-Step Plus RT-PCR Kit III
|
RP01-III-Plus
|
50r
|
$4500
|
|
One-Step RT-PCR Kit IV
|
RP01-IV
|
50r/250r
|
$4500/19130
|
|
One-Step Plus RT-PCR Kit IV
|
RP01-IV-Plus
|
50r
|
$4800
|
|
One-Step RT-PCR II with Dye
|
RP01D-II
|
50r
|
$4980
|
Two Step RT-PCR Kit
|
Product Name
|
Cat. No.
|
Package
|
Price
|
|
Two-Step RT-PCR kit III (SuperSAMscriptase III/GenHot)
|
RP012-M3
|
50r
|
$4500
|
|
Two-Step RT-PCR kit IV (SuperSAMscriptease IV/GbeHot)
|
RP012-M4
|
50r
|
$5000
|
One-Step RT-qPCR Kit
|
Product
|
Cat. No.
|
Enzyme
|
|
One-Step BrightGreen RT-qPCR Kit (with low ROX)
|
QRP01BG-LR01
|
SuperSAMscript III/ Hot-start Taq
|
|
One-Step BrightGreen RT-qPCR Kit (with high ROX)
|
QRP01BG-HR01
|
SuperSAMscript III/ Hot-start Taq
|
|
One-Step BrightGreen RT-qPCR Kit (without ROX)
|
QRP01BG-NR01
|
SuperSAMscript III/ Hot-start Taq
|
|
Product Name
|
Cat. No.
|
Package
|
Price
|
|
Green One-Step qRT-PCR Kit III
|
QRP01-100
|
100r
|
$4800
|
|
One-Step qRT-PCR Kit III（Probe)
|
QRP01P-100
|
100r
|
$5400
|
qPCR Master Mix
|
|
產品名稱
|
產品編號
|
包裝
|
價格($NT)
|
|
qPCR
Master Mix
|
GM SYBR qPCR Kit (2X), (High ROX)
|
QPSY01
|
1 ml
|
1000
|
|
GM SYBR qPCR Kit (2X), (w/o ROX)
|
QPSY02
|
1 ml
|
1000
|
|
GM SYBR qPCR Kit (2X), (Low ROX)
|
QPSY03
|
1 ml
|
1000
|
|
GM Fast SYBR qPCR Kit (High ROX),
|
QPSY04-1
|
1 ml
|
1400
|
|
GM Fast SYBR qPCR Kit (Low ROX),
|
QPSY05-1
|
1 ml
|
1400
|
|
GM qPCR Master Mix (Probe),
|
QPSY01P-1
|
1 ml
|
1600
|
|
GM Fast qPCR Master Mix (Probe)
|
QPSY04P-1
|
1 ml
|
1600
|
|
BrightGreen 2X qPCR MasterMix-Low ROX
|
QPBG01-LR01
|
1 ml
|
1200
|
|
BrightGreen 2X qPCR MasterMix- ROX
|
QPBG01-IR01
|
1 ml
|
1200
|
|
BrightGreen 2X qPCR MasterMix-No dye
|
QPBG01-N01
|
1 ml
|
1200
|
PCR Cloning Kits & DNA Ligation Kits
TopoFast PCR Cloning Kit
- Ligation只要 5 min
- Blunt end or A-end 的 PCR產物都能連接
- 特殊工藝,不需籃白篩
- Gene expression,可直接選殖到可以表現的 Topo 載體
- 不需要限制酵素
- 不需要 T4 Ligase
-
|
Cat. #
|
Product
|
Note
|
|
GBM16A
|
pBM16A TOPOFast PCR Cloning Kit, 20r
|
General TA Vector
|
|
GBM23
|
pBM23 TOPOFast PCR Cloning Kit, 20r
|
General TA Vector(including Restriction size)
|
|
GBM30
|
pBM30 TOPOFast PCR Cloning Kit, 20r
|
Gene expression Vector (prokaryotic)
|
|
GBM40
|
pBM40 TOPOFast PCR Cloning Kit, 20r
|
Gene expression Vector (Eukaryotic)
|
GM Seamless Cloning Kit 無縫選殖試劑盒
產品介紹：
區別於TOPO Cloning、TA Cloning 和 T4 Ligase (pGEM-T)連接等常規選殖方法，無縫選殖(Seamless Cloning)s技術可在重組?的作用下，只需一步反應，便可將片段克隆到任何載體中的任意位置得到重組質粒。無縫選殖技術作為一種非常強大的選殖技術，具有快速、簡便、高效、多片段組裝和定向克隆等特點，用於單個 DNA 片段的克隆，多個 DNA 片段組裝克隆以及多位點突變構建等實驗目的。
產品特點：
1.簡單、快速、精確、定向克隆，連接過程只需要15分鐘。
2.只需要簡單的PCR擴增就可以製備目的片段，不需要內切?、連接?和磷酸化?。
3.線性化載體的製備可以通過PCR擴增或選擇合適的內切??切。
4.可以克隆長片段DNA。
|
DESCRIPTION
|
Cat.No.
|
REACTION
|
|
GM Seamless Cloning Kit, 20r
|
GMS116
|
20r
|
|
pUC57-simpleEVL Seamless cloning kit, 20r
|
GMS120
|
20r
|
|
2× GM SeamLess Mix, 20r
|
GMS117
|
20r
|
|
| |
|
商 品 其 它 備 註 |
 |
|
|
