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GM/ RP PCR 系列相關產品 |
人氣指數 |
3792 |
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| 型號：GM/ RP |
| 中文名稱：PCR 系列相關產品 |
| 英文名稱： |
| 廠牌：GeneMark |
| 供應商：賽恩斯生物科技股份有限公司 |
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商 品 說 明 資 料 |
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- First Stranded cDNA Synthesis Kit
DNA Polymerases
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Product Name
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GenTaq DNA Polymerase
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GenTaq Plus DNA Polymerase
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GenHot Taq DNA Polymerase
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GenFast Taq DNA Polymerase
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gmPfu DNA Polymerase
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LongFast Pfu DNA Polymerase
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GenBest Taq DNA Polymerase
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Polymerase
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GenTaq
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GenTaq + Pfu
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Taq + Mab
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Modified Taq
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Modified Pfu
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Modified Pfu
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Modified Pfu
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Proofreading
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No
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Yes
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No
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No
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Yes
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Yes
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Yes
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Product Overhang
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3’-A
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3’-A
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3’-A
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3’-A
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Blunt
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Blunt
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Blunt
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Extension
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1 min/kb
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1 min/kb
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1 min/kb
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15 sec/kb
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1 min/kb
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15-30 sec/kb
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10~30 sec/kb
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PCR product size
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Up to 14 kb
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Up to 14 kb
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Up to 6 kb
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Up to 15 kb
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Up to 10 kb
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Up to 10 KB
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Up to 20 KB
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Application
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Detection
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Detection Cloning Expression
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Detection Multiplex PCR
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Fast PCR Detection Multiplex PCR
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Cloning Expression Mutagenesis
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Fast PCR Cloning Expression Mutagenesis
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Fast PCR Cloning Expression Mutagenesis
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產品名稱
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產品編號
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包裝
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價格($NT)
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PCR Polymerase
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GenTaq DNA Polymerase
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GM008
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1000 U (2U/ul or 5U/ul)
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1890
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GenTaq Plus DNA Polymerase
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GM008P
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250 U
1000 U
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2048
8190
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GenHot Taq DNA Polymerase
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GM008HS
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200 U
1000 U
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1800
7200
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GenFast Taq DNA Polymerase
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GM008F
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200 U
1000 U
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1200
4800
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gmPfu DNA Polymerase
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GPU425
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250U
500U
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1890
3402
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LongFast Pfu DNA Polymerase
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GPU4
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200U
1000U
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3200
13200
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GenBest Taq DNA Polymerase
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GM008B
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200U
1000U
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2400
9600
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產品名稱
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產品編號
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包裝
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價格($NT)
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PCR Master Mix
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PCR Master Mix (2X)
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RP02
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400r
2000r
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2079
8316
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PCR Master Mix II
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RP02 II
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400r
2000r
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2079
8316
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PCR Plus Master Mix II
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RP02-Plus-II
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100r
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2394
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PCR Pfu Master Mix II
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RP02-pfu-II
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200r
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2520
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PCR Hot-Start Master Mix II
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RP02HS-II
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200r
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3150
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PCR Dye Master Mix
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PCR Dye Master Mix II (2X)
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RP02D-II-B
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200r
1000r
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1155
4620
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PCR Dye Master Mix II (5X)
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RP02D-II-A
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200r
1000r
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1155
4620
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2X GenFast PCR MasterMix, 1ml
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RP02D-Fast
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100r
500r
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1800
9000
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PCR Dye Plus Master Mix
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RP02D-Plus
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100r
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2394
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PCR Dye Pfu Master Mix
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RP02D-pfu
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200r
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2520
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PCR Dye Hot-Start Master Mix
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RP02D-HS
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200r
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3150
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Cat. No.
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Product
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Sample
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DNA Yield
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Format
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Operation Time
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RP02-LD300
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SAMtissuet Direct PCR Kit
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cells, mouse tails, liver,muscle, hair shafts, skin, saliva and other animal tissues
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For PCR
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no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
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15 min
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RP02-LD301
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SAMbacteria Direct PCR Kit
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both gram-positive and gram-negative (genomic DNA and plasmid DNA)
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For PCR
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no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
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-
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RP02-LD303
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SAMseed Direct PCR Kit
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different kinds of seeds ; Arabidopsis, carrot, corn, cucumber, pepper, soybean, turnip, wheat and more
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For PCR
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no DNA purification needed ; Uses pre- 2X PCR mix( containing dye)
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15 min
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RP02-LD304
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SAMblood Direct PCR Kit
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blood, saliva, other body, fluids, buccal cells on swabs, Cultured cells, and other liquid cells
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For PCR
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no DNA purification ; Uses pre-optimized 2X PCR mix( containing dye)
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15 min
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RP02-PD8
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GMplant Direct PCR Kit
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plant leaves
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For PCR
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no DNA purification needed ; Uses pre-optimized 2X PCR mix( containing dye)
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-
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產品名稱
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產品編號
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包裝
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價格($NT)
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SAMtissue Direct PCR Kit
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RP02-LD300
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100r
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5460
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SAMbacteria Direct PCR Kit
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RP02-LD301
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100r
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5460
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GMplant Direct PCR Kit
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RP02-PD801
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100r
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5460
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SAMseed Direct PCR Kit
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RP02-LD303
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100r
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5460
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SAMblood Direct PCR Kit
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RP02-LD304
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100r
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5460
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Reverse transcription-PCR (RT-PCR)
> Transcriptase 反轉錄?
> 一步法RT-PCR
> 兩步法RT-PCR
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AMV
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MMLV
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來源
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Avian
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Murine
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最適作用溫度
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42℃ (可有效解開二級結構)
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37℃
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RNase H
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強
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弱
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合成長度
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短
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長
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** MMLV reverse transcription 改良版 → 提高溫度耐受性
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SuperSAMscript III Reverse Transcriptase
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SuperSAMscript IV Reverse Transcriptase
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作用溫度
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37℃~55℃ (適用於具有複雜二級結構之RNA)
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42℃~65℃ (適用於具有複雜二級結構之RNA)
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合成片段大小
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100bp~15kb（無RNase H活性, 可增加transcripts產量）
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100bp~12kb (適合長片段cDNA合成)
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模板量
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Total RNA: 1 ng-5 μg mRNA: 1 ng-250 ng Specific RNA: 0.01 pg-0.5 μg
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Total RNA 10 ng~5 μg mRNA 1ng~500 ng Specific RNA: 0.01 pg-0.5 μg
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First-Stranded cDNA Synthesis Kit
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Product Name
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Cat. No.
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Package
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Price
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SuperSAMscript III First Stranded cDNA Synthesis Kit
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GRS03M
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50r
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$3000
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SuperSAMscript IV First Stranded cDNA Synthesis Kit
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GRS04M
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50r
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$3600
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One-Step RT-PCR Kit
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Product Name
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Cat. No.
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Package
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Price
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One-Step RT-PCR Kit III
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RP01-III
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50r/250r
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$3900/$16580
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One-Step Plus RT-PCR Kit III
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RP01-III-Plus
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50r
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$4500
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One-Step RT-PCR Kit IV
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RP01-IV
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50r/250r
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$4500/19130
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One-Step Plus RT-PCR Kit IV
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RP01-IV-Plus
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50r
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$4800
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One-Step RT-PCR II with Dye
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RP01D-II
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50r
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$4980
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Two Step RT-PCR Kit
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Product Name
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Cat. No.
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Package
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Price
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Two-Step RT-PCR kit III (SuperSAMscriptase III/GenHot)
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RP012-M3
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50r
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$4500
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Two-Step RT-PCR kit IV (SuperSAMscriptease IV/GbeHot)
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RP012-M4
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50r
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$5000
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One-Step RT-qPCR Kit
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Product
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Cat. No.
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Enzyme
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One-Step BrightGreen RT-qPCR Kit (with low ROX)
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QRP01BG-LR01
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SuperSAMscript III/ Hot-start Taq
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One-Step BrightGreen RT-qPCR Kit (with high ROX)
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QRP01BG-HR01
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SuperSAMscript III/ Hot-start Taq
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One-Step BrightGreen RT-qPCR Kit (without ROX)
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QRP01BG-NR01
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SuperSAMscript III/ Hot-start Taq
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Product Name
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Cat. No.
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Package
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Price
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Green One-Step qRT-PCR Kit III
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QRP01-100
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100r
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$4800
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One-Step qRT-PCR Kit III（Probe)
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QRP01P-100
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100r
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$5400
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qPCR Master Mix
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產品名稱
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產品編號
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包裝
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價格($NT)
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qPCR
Master Mix
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GM SYBR qPCR Kit (2X), (High ROX)
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QPSY01
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1 ml
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1000
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GM SYBR qPCR Kit (2X), (w/o ROX)
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QPSY02
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1 ml
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1000
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GM SYBR qPCR Kit (2X), (Low ROX)
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QPSY03
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1 ml
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1000
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GM Fast SYBR qPCR Kit (High ROX),
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QPSY04-1
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1 ml
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1400
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GM Fast SYBR qPCR Kit (Low ROX),
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QPSY05-1
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1 ml
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1400
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GM qPCR Master Mix (Probe),
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QPSY01P-1
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1 ml
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1600
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GM Fast qPCR Master Mix (Probe)
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QPSY04P-1
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1 ml
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1600
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BrightGreen 2X qPCR MasterMix-Low ROX
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QPBG01-LR01
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1 ml
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1200
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BrightGreen 2X qPCR MasterMix- ROX
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QPBG01-IR01
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1 ml
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1200
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BrightGreen 2X qPCR MasterMix-No dye
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QPBG01-N01
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1 ml
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1200
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PCR Cloning Kits & DNA Ligation Kits
TopoFast PCR Cloning Kit
- Ligation只要 5 min
- Blunt end or A-end 的 PCR產物都能連接
- 特殊工藝,不需籃白篩
- Gene expression,可直接選殖到可以表現的 Topo 載體
- 不需要限制酵素
- 不需要 T4 Ligase
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Cat. #
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Product
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Note
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GBM16A
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pBM16A TOPOFast PCR Cloning Kit, 20r
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General TA Vector
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GBM23
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pBM23 TOPOFast PCR Cloning Kit, 20r
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General TA Vector(including Restriction size)
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GBM30
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pBM30 TOPOFast PCR Cloning Kit, 20r
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Gene expression Vector (prokaryotic)
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GBM40
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pBM40 TOPOFast PCR Cloning Kit, 20r
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Gene expression Vector (Eukaryotic)
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GM Seamless Cloning Kit 無縫選殖試劑盒
產品介紹：
區別於TOPO Cloning、TA Cloning 和 T4 Ligase (pGEM-T)連接等常規選殖方法，無縫選殖(Seamless Cloning)s技術可在重組?的作用下，只需一步反應，便可將片段克隆到任何載體中的任意位置得到重組質粒。無縫選殖技術作為一種非常強大的選殖技術，具有快速、簡便、高效、多片段組裝和定向克隆等特點，用於單個 DNA 片段的克隆，多個 DNA 片段組裝克隆以及多位點突變構建等實驗目的。
產品特點：
1.簡單、快速、精確、定向克隆，連接過程只需要15分鐘。
2.只需要簡單的PCR擴增就可以製備目的片段，不需要內切?、連接?和磷酸化?。
3.線性化載體的製備可以通過PCR擴增或選擇合適的內切??切。
4.可以克隆長片段DNA。
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DESCRIPTION
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Cat.No.
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REACTION
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GM Seamless Cloning Kit, 20r
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GMS116
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20r
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pUC57-simpleEVL Seamless cloning kit, 20r
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GMS120
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20r
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2× GM SeamLess Mix, 20r
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GMS117
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20r
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