Ultracentrifuge-Independent Method Separate Four Subcellular Protein Fractions
FracPRO™ Protein Fractionation Kit is a product with highly specialized fractionation buffers which can target specific subcellular compartment and with ultracentrifuge-independent method to separate four subcellular protein fractions, including nuclear, cytosol, membrane/organelle, and cytoskeletal fractions in a detergent-based procedure. It can simultaneously preserve the structural integrity of the proteins and can directly be used in SDS-PAGE, enzyme analyses, gel mobility shift assays and other procedures.
Special Characteristic
- Compatible : Variable sample application including cultured cells and mammalian tissues
- Quick : Separate and collect four subcellular protein fractions in short time
- Simple : Isolate high quality protein fraction with easy procedure
Product Detail :
Figure 1. The fractionation efficacy of Human leukemia cell line (K562) by FracPRO™ Protein Fractionation Kit.
The showed result detected by Western blot. (A) Heat shock protein 70 antibody (B) ATPase antibody (C) Histone deacetylase 2 antibody (D) Vimentin antibody. M: Pre-stained protein marker; Cy.: cytosol fraction; Mem.: membrane / organelle fraction; Nu.: nuclear fraction; Sk.: cytoskeletal fraction.
Order Information
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Cat. No.
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Product Name
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Description
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FP01-5
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FracPRO™ Protein Fractionation Kit
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10ml Cytosol buffer +
10ml Membrane/Organelle buffer + 7.5ml Nuclear buffer + 1.5ml Cytoskeletal buffer
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FP01-50
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FracPRO™ Protein Fractionation Kit
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100ml Cytosol buffer +100ml Membrane/Organelle buffer +75ml Nuclear buffer + 15ml Cytoskeletal buffer
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