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Product Detail :
Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™.
(A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.
Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™
30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.
Figure 3. Evaluate ECL substrate efficacy by LuminolPen™.
30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. Signal result present by normal ECL substrate is stronger than using expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiment to similar experimental condition.
Order Information :
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Cat. No
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Product Namber
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Discription
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LH03-50
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LuminolPen™, HRP System
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About 1,000 membranes Drawing
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LH03-10
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LuminolPen™, HRP System
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About 100 membranes Drawing
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