BlockPRO 西方墨點法遮蓋液成份單純，能有效阻斷實驗中可 能產生非專一性結合的位置，不會遮蓋抗原，提高抗原與抗體 結合之專一性。
產品特點
● 優異遮蔽：可降低背景雜訊，使目標蛋白更清晰
● 無 biotin：適用於biotin-avidin系統
● 無磷酸化蛋白：適用於偵測磷酸化蛋白
Product Detail :
Figure 1. Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer.
Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection : Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.
Figure 2. Blocking with skim milk or BlockPRO™ Blocking Buffer and detect by SEM.
30 μg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or BlockPRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.
訂購資訊 :
| Cat. No. |
Product Name |
Description |
| BP01-1L |
BlockPRO™ Blocking Buffer |
1 kit 500mL Solution X 2
|
