The GM SYBR qPCR Kit is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixtures contain Hot StartTaq DNA polymeraseand other components for PCR with the exception of template and primers. Hot Start Taq DNA polymerase is complex mixture of a thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerase. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The GM SYBR qPCRMix is supplied as a 2X concentrated ready-to-use mix that is a 1X mixture of recombinant 2 U of Hot Start Taq DNA polymerase, reaction buffer, 3 mM MgCl₂, 250 μM dNTP, SYBR Green I, ROX passive reference dye, and enzyme stabilizer sufficient to allow efficient amplification of template in 25 μl PCR.
    

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GM SYBR qPCR Kit (without ROX)