TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.
產品資訊
《快速簡單》
- TOPO cloning 系統
- 不需使用Ligase反應，僅需5分鐘即可完成 ligation反應。
- 使用Ampicillin、藍白篩選。《好用、高效率》
- Insert 長度至少可達3 kb
《好用、高效率》
- 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen-KB DNA Ladder
TOPfast Vector Information
產品內容物
| |
|
| 1 vial of 20 μl |
TOPfast Vector (10 ng/μl) |
| 1 vial of 20 μl |
6X TOPfastTM Buffer |
| 1 vial of 5 μl |
Control insert DNA (1 kb) |
| 21 vials of 100 μl |
DH5α Competent Cell |
| 1 vials 5 μl |
Control Plasmid |
| 1 vial of 400 μl |
M13 Control primer mix |
| 1 vial of 7.5 ml |
SOC medium |
| 1 vial of 1 ml |
Sterile water |
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| Products: |
Cat. No. |
Size |
|
TOPfast PCR Cloning Kit
(Including competent cell)
|
GTOP01-20
GTOP01-40
|
20 r
40 r
|
|
TOPfast PCR Cloning Vector
(not including competent cell)
|
GTOP01-V20
GTOP01-V40
|
20 r
40 r
|
|
