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《快速簡單》
- TOPO cloning 系統
- 不需使用Ligase反應，僅需5分鐘即可完成 ligation反應。
- 不需額外做A-tailing
- 使用Ampicillin、藍白篩選。
《好用、高效率》
- Insert 長度至少可達3 kb
- 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl) , 1 kb (50 ng/µl), 2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPBlunt PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen100 DNA Ladder
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