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DP012-50, DP012-300 ²Ä¤G¥N½èÅé DNA ¯Â¤Æ¸Õ¾¯²Õ. |
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| «¬¸¹¡GDP012-50, DP012-300 |
| ¤¤¤å¦WºÙ¡G²Ä¤G¥N½èÅé DNA ¯Â¤Æ¸Õ¾¯²Õ. |
| ^¤å¦WºÙ¡GPlasmid Miniprep Purification Kit II |
| ¼tµP¡GGeneMark |
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A. ²³æ®e©ö: ¨Ï¥Îspin column ¤èªk¡C
B. ²£¶q¦h: ¨C¤@¤ÏÀ³¥i±q1-5 ml culture ¯Â¤Æ40 μg ultra pure plasmid DNA¡C
C. ¯Â«×°ª: ¯Â¤Æ§¹¤§DNA ¯Â«×°ª, ¥iª½±µautomatic sequencing, in vitro transcription, PCR,enzyme digestion µ¥¸ÕÅç¡C
D. §Ö³t: ¹Lµ{10 ¡ã 15 ¤ÀÄÁ¡C
E. ¿W¯S°t¤è: ¦³®Ä¥h°£endonuclease¡A°w¹ïEndA+ µßºØBL21(DE3) HB101 ©MJM109 ¯S§O¦³®Ä¡C
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